Salicylate and catechol levels are maintained in nahG transgenic poplar.
Identifieur interne : 003A79 ( Main/Exploration ); précédent : 003A78; suivant : 003A80Salicylate and catechol levels are maintained in nahG transgenic poplar.
Auteurs : Alison M. Morse [États-Unis] ; Timothy J. Tschaplinski ; Christopher Dervinis ; Paula M. Pijut ; Eric A. Schmelz ; Wendy Day ; John M. DavisSource :
- Phytochemistry [ 0031-9422 ] ; 2007.
Descripteurs français
- KwdFr :
- ARN des plantes (génétique), Catéchols (isolement et purification), Catéchols (métabolisme), Mixed function oxygenases (biosynthèse), Mixed function oxygenases (génétique), Populus (enzymologie), Populus (génétique), Populus (métabolisme), Protéines bactériennes (biosynthèse), Protéines bactériennes (génétique), Salicylates (isolement et purification), Salicylates (métabolisme), Technique de Northern (MeSH), Transgènes (MeSH), Végétaux génétiquement modifiés (enzymologie), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- biosynthèse : Mixed function oxygenases, Protéines bactériennes.
- enzymologie : Populus, Végétaux génétiquement modifiés.
- génétique : ARN des plantes, Mixed function oxygenases, Populus, Protéines bactériennes, Végétaux génétiquement modifiés.
- isolement et purification : Catéchols, Salicylates.
- métabolisme : Catéchols, Populus, Salicylates, Végétaux génétiquement modifiés.
- Technique de Northern, Transgènes.
English descriptors
- KwdEn :
- Bacterial Proteins (biosynthesis), Bacterial Proteins (genetics), Blotting, Northern (MeSH), Catechols (isolation & purification), Catechols (metabolism), Mixed Function Oxygenases (biosynthesis), Mixed Function Oxygenases (genetics), Plants, Genetically Modified (enzymology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (enzymology), Populus (genetics), Populus (metabolism), RNA, Plant (genetics), Salicylates (isolation & purification), Salicylates (metabolism), Transgenes (MeSH).
- MESH :
- chemical , biosynthesis : Bacterial Proteins, Mixed Function Oxygenases.
- chemical , genetics : Bacterial Proteins, Mixed Function Oxygenases, RNA, Plant.
- chemical , isolation & purification : Catechols, Salicylates.
- chemical , metabolism : Catechols, Salicylates.
- enzymology : Plants, Genetically Modified, Populus.
- genetics : Plants, Genetically Modified, Populus.
- metabolism : Plants, Genetically Modified, Populus.
- Blotting, Northern, Transgenes.
Abstract
Metabolic profiling was used to investigate the molecular phenotypes of a transgenic Populus tremula x P. alba hybrid expressing the nahG transgene, a bacterial gene encoding salicylate hydroxylase that converts salicylic acid to catechol. Despite the efficacy of this transgenic approach to reduce salicylic acid levels in other model systems and thereby elucidate roles for salicylic acid in plant signaling, transgenic poplars had similar foliar levels of salicylic acid and catechol to that of non-transformed controls and exhibited no morphological phenotypes. To gain a deeper understanding of the basis for these observations, we analyzed metabolic profiles of leaves as influenced by transgene expression. Expression of nahG decreased quinic acid conjugates and increased catechol glucoside, while exerting little effect on levels of salicylic acid and catechol, the substrate and product, respectively, of the nahG enzyme. This suggests a biological role of elevated constitutive salicylic acid levels in Populus, in contrast to other plant systems in which nahG dramatically reduces salicylic acid levels.
DOI: 10.1016/j.phytochem.2007.05.014
PubMed: 17599371
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Tschaplinski</name></author><author><name sortKey="Dervinis, Christopher" sort="Dervinis, Christopher" uniqKey="Dervinis C" first="Christopher" last="Dervinis">Christopher Dervinis</name></author><author><name sortKey="Pijut, Paula M" sort="Pijut, Paula M" uniqKey="Pijut P" first="Paula M" last="Pijut">Paula M. Pijut</name></author><author><name sortKey="Schmelz, Eric A" sort="Schmelz, Eric A" uniqKey="Schmelz E" first="Eric A" last="Schmelz">Eric A. Schmelz</name></author><author><name sortKey="Day, Wendy" sort="Day, Wendy" uniqKey="Day W" first="Wendy" last="Day">Wendy Day</name></author><author><name sortKey="Davis, John M" sort="Davis, John M" uniqKey="Davis J" first="John M" last="Davis">John M. Davis</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17599371</idno><idno type="pmid">17599371</idno><idno type="doi">10.1016/j.phytochem.2007.05.014</idno><idno type="wicri:Area/Main/Corpus">003B23</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003B23</idno><idno type="wicri:Area/Main/Curation">003B23</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003B23</idno><idno type="wicri:Area/Main/Exploration">003B23</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Salicylate and catechol levels are maintained in nahG transgenic poplar.</title><author><name sortKey="Morse, Alison M" sort="Morse, Alison M" uniqKey="Morse A" first="Alison M" last="Morse">Alison M. Morse</name><affiliation wicri:level="2"><nlm:affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL 32611, USA. ammorse@ufl.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL 32611</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name></author><author><name sortKey="Dervinis, Christopher" sort="Dervinis, Christopher" uniqKey="Dervinis C" first="Christopher" last="Dervinis">Christopher Dervinis</name></author><author><name sortKey="Pijut, Paula M" sort="Pijut, Paula M" uniqKey="Pijut P" first="Paula M" last="Pijut">Paula M. Pijut</name></author><author><name sortKey="Schmelz, Eric A" sort="Schmelz, Eric A" uniqKey="Schmelz E" first="Eric A" last="Schmelz">Eric A. Schmelz</name></author><author><name sortKey="Day, Wendy" sort="Day, Wendy" uniqKey="Day W" first="Wendy" last="Day">Wendy Day</name></author><author><name sortKey="Davis, John M" sort="Davis, John M" uniqKey="Davis J" first="John M" last="Davis">John M. Davis</name></author></analytic><series><title level="j">Phytochemistry</title><idno type="ISSN">0031-9422</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (biosynthesis)</term><term>Bacterial Proteins (genetics)</term><term>Blotting, Northern (MeSH)</term><term>Catechols (isolation & purification)</term><term>Catechols (metabolism)</term><term>Mixed Function Oxygenases (biosynthesis)</term><term>Mixed Function Oxygenases (genetics)</term><term>Plants, Genetically Modified (enzymology)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>RNA, Plant (genetics)</term><term>Salicylates (isolation & purification)</term><term>Salicylates (metabolism)</term><term>Transgenes (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (génétique)</term><term>Catéchols (isolement et purification)</term><term>Catéchols (métabolisme)</term><term>Mixed function oxygenases (biosynthèse)</term><term>Mixed function oxygenases (génétique)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines bactériennes (biosynthèse)</term><term>Protéines bactériennes (génétique)</term><term>Salicylates (isolement et purification)</term><term>Salicylates (métabolisme)</term><term>Technique de Northern (MeSH)</term><term>Transgènes (MeSH)</term><term>Végétaux génétiquement modifiés (enzymologie)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Bacterial Proteins</term><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Mixed Function Oxygenases</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Catechols</term><term>Salicylates</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Catechols</term><term>Salicylates</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Mixed function oxygenases</term><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term><term>Mixed function oxygenases</term><term>Populus</term><term>Protéines bactériennes</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Catéchols</term><term>Salicylates</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Catéchols</term><term>Populus</term><term>Salicylates</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Northern</term><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Technique de Northern</term><term>Transgènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Metabolic profiling was used to investigate the molecular phenotypes of a transgenic Populus tremula x P. alba hybrid expressing the nahG transgene, a bacterial gene encoding salicylate hydroxylase that converts salicylic acid to catechol. Despite the efficacy of this transgenic approach to reduce salicylic acid levels in other model systems and thereby elucidate roles for salicylic acid in plant signaling, transgenic poplars had similar foliar levels of salicylic acid and catechol to that of non-transformed controls and exhibited no morphological phenotypes. To gain a deeper understanding of the basis for these observations, we analyzed metabolic profiles of leaves as influenced by transgene expression. Expression of nahG decreased quinic acid conjugates and increased catechol glucoside, while exerting little effect on levels of salicylic acid and catechol, the substrate and product, respectively, of the nahG enzyme. This suggests a biological role of elevated constitutive salicylic acid levels in Populus, in contrast to other plant systems in which nahG dramatically reduces salicylic acid levels.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17599371</PMID><DateCompleted><Year>2007</Year><Month>10</Month><Day>04</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-9422</ISSN><JournalIssue CitedMedium="Print"><Volume>68</Volume><Issue>15</Issue><PubDate><Year>2007</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Phytochemistry</Title><ISOAbbreviation>Phytochemistry</ISOAbbreviation></Journal><ArticleTitle>Salicylate and catechol levels are maintained in nahG transgenic poplar.</ArticleTitle><Pagination><MedlinePgn>2043-52</MedlinePgn></Pagination><Abstract><AbstractText>Metabolic profiling was used to investigate the molecular phenotypes of a transgenic Populus tremula x P. alba hybrid expressing the nahG transgene, a bacterial gene encoding salicylate hydroxylase that converts salicylic acid to catechol. Despite the efficacy of this transgenic approach to reduce salicylic acid levels in other model systems and thereby elucidate roles for salicylic acid in plant signaling, transgenic poplars had similar foliar levels of salicylic acid and catechol to that of non-transformed controls and exhibited no morphological phenotypes. To gain a deeper understanding of the basis for these observations, we analyzed metabolic profiles of leaves as influenced by transgene expression. Expression of nahG decreased quinic acid conjugates and increased catechol glucoside, while exerting little effect on levels of salicylic acid and catechol, the substrate and product, respectively, of the nahG enzyme. This suggests a biological role of elevated constitutive salicylic acid levels in Populus, in contrast to other plant systems in which nahG dramatically reduces salicylic acid levels.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Morse</LastName><ForeName>Alison M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL 32611, USA. ammorse@ufl.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tschaplinski</LastName><ForeName>Timothy J</ForeName><Initials>TJ</Initials></Author><Author ValidYN="Y"><LastName>Dervinis</LastName><ForeName>Christopher</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Pijut</LastName><ForeName>Paula M</ForeName><Initials>PM</Initials></Author><Author ValidYN="Y"><LastName>Schmelz</LastName><ForeName>Eric A</ForeName><Initials>EA</Initials></Author><Author ValidYN="Y"><LastName>Day</LastName><ForeName>Wendy</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Davis</LastName><ForeName>John M</ForeName><Initials>JM</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>06</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochemistry</MedlineTA><NlmUniqueID>0151434</NlmUniqueID><ISSNLinking>0031-9422</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002396">Catechols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012459">Salicylates</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D006899">Mixed Function Oxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.13.1</RegistryNumber><NameOfSubstance UI="C022619">salicylate 1-monooxygenase</NameOfSubstance></Chemical><Chemical><RegistryNumber>LF3AJ089DQ</RegistryNumber><NameOfSubstance UI="C034221">catechol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015152" MajorTopicYN="N">Blotting, Northern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002396" MajorTopicYN="N">Catechols</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006899" MajorTopicYN="N">Mixed Function Oxygenases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="Y">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="Y">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012459" MajorTopicYN="N">Salicylates</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>09</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2007</Year><Month>04</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2007</Year><Month>05</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>6</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>10</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>6</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17599371</ArticleId><ArticleId IdType="pii">S0031-9422(07)00328-7</ArticleId><ArticleId IdType="doi">10.1016/j.phytochem.2007.05.014</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><noCountry><name sortKey="Davis, John M" sort="Davis, John M" uniqKey="Davis J" first="John M" last="Davis">John M. Davis</name><name sortKey="Day, Wendy" sort="Day, Wendy" uniqKey="Day W" first="Wendy" last="Day">Wendy Day</name><name sortKey="Dervinis, Christopher" sort="Dervinis, Christopher" uniqKey="Dervinis C" first="Christopher" last="Dervinis">Christopher Dervinis</name><name sortKey="Pijut, Paula M" sort="Pijut, Paula M" uniqKey="Pijut P" first="Paula M" last="Pijut">Paula M. Pijut</name><name sortKey="Schmelz, Eric A" sort="Schmelz, Eric A" uniqKey="Schmelz E" first="Eric A" last="Schmelz">Eric A. Schmelz</name><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name></noCountry><country name="États-Unis"><region name="Floride"><name sortKey="Morse, Alison M" sort="Morse, Alison M" uniqKey="Morse A" first="Alison M" last="Morse">Alison M. Morse</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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